衰老的代谢控制 Metabolic Control and Aging Lab

点击次数：

研究室负责人(PI)：

向阳 教授，博士生导师

研究方向：研究主要集中于衰老的代谢控制的研究。结合百岁老人遗传分析（全基因组关联分析及GWAS）以及线虫模型，建立了“百岁老人遗传-表型资源库”。结合前期的天然产物研究，建立了“衰老干预小分子资源库”。通过两个特色库的交叉研究，以及线虫和小鼠等模式生物验证和机制研究，期望发掘一批能够调控机体代谢，延缓衰老，促进健康老龄化的药物先导分子。

主持科研项目

1. 组织器官衰老顺序影响个体亚健康及疾病的机制，科技部重点研发计划，项目编号：2023YFC3603301（课题一骨干、子课题负责人，课题负责人田小利），105万元，2023.12-2026.11.

2. 运动-营养改善代谢稳态、延缓衰老、促进健康的机制，科技部重点研发计划，项目编号2020YFC2002903（子课题负责人，项目协调人），支配经费108万元，2020.06-2023.06.

3. 江西省XXXX人才资助计划，项目编号20192BCB23003，资助金额30万元，2019.01-2022.12。

4. 抑素蛋白和凝血酶受体协同调节内皮细胞衰老的机制，国家自然科学基金面上项目，项目编号81873814，直接经费61万元，2019.01-2022.12。

5. 肠道菌群孔道形成毒素通过调控线粒体功能影响脂代谢的机制研究，江西省杰出青年基金项目（青年重点项目），项目编号2018ACB21036，经费20万元，2018.01-2021.12

6. 肠道菌群孔道形成毒素与三叶因子复合物在血管内皮衰老中的作用机制研究，国家自然科学基金重大研究计划培育项目，项目编号91649120，直接经费60万元，2017.01-2019.12。

7. 滇产野菊花防治皮肤光损伤的有效成分分离及机制研究，遗传资源与进化国家重点实验室开放基金，项目编号GREKF18-10，资助金额7万元，2018年6月至2021年5月。

8. 两栖动物孔道形成毒素类似蛋白在抗感染免疫中的作用，中国科学院西部之光人才一般项目，资助金额30万元。2015.01-2017.12

9. 斑马鱼aerolysin毒素类似蛋白zebralysin在抗感染免疫中的作用，云南省自然科学基金一般项目，项目编号2014FB175，资助金额：10万元。2015.01-2017.12

10. 非晶状体晶状体蛋白与三叶因子复合物在大蹼铃蟾抗感染免疫中的作用研究，国家自然科学基金青年基金，项目编号31301884，资助金额：24万元，2014.01-2016.12。

发表论文

第一及通讯作者文章

2023

Hui-Jie Zhang#, Ben-Hui Wang#, Xiang Wang#, Chun-Ping Huang#, Si-Man Xu, Jia-Li Wang, Tian-E Huang, Wan-Li Xiao, Xiao-Li Tian, Xin-Qiang Lan*, Qi-Quan Wang* & Yang Xiang*, Handelin alleviates cachexia- and aging-induced skeletal muscle atrophy by improving protein homeostasis and inhibiting inflammation,  Journal of Cachexia, Sarcopenia and Muscle 2023 15 (1), 173-188

Huijie Zhang, Weirong Zeng, Ming-ming Zhao, Jiali Wang, Qiquan Wang, Ting Chen, Yuyan Zhang, Wenhui Lee, Shenghan Chen, Yun Zhang*, Xinqiang Lan*, Yang Xiang*, Caenorhabditis elegans LIN‐24, a homolog of bacterial pore‐forming toxin, protects the host from microbial infection. The FASEB Journal. 2023. DOI:10.1096/fj.202300063R. (MEDLINE:37682220)

Huang, T.; Zhou, J.; Wang, B.; Wang, X.; Xiao, W.; Yang, M.; Liu, Y.; Wang, Q.; Xiang, Y.*; Lan, X*. Integrated Amino Acids and Transcriptome Analysis Reveals Arginine Transporter SLC7A2 Is a Novel Regulator of Myogenic Differentiation.  Int. J. Mol. Sci. 2024, 25, 95.

Qi-Quan Wang, Xin-Qiang Lan, Xue-Song Wei, Si-Man Xu, Ling-Zhen Liu, Xian-Ling Bian, Lin Zeng, Xiao-Long Guo, Ying-Qi Guo, Wen-Hui Lee, Yang Xiang*, Yun Zhang*. Amphibian pore-forming protein βγ-CAT drives metabolite release from small extracellular vesicles through channel formation. Zoological Research, 2023, 44(4): 739-742. doi: 10.24272/j.issn.2095-8137.2022.510

Yang Xiang*, Qi-Quan Wang, Xin-Qiang Lan, Hui-Jie Zhang, Dai-Xu Wei*. Function and treatment strategies of β-hydroxybutyrate in aging.  Smart Materials in Medicine . 2023;4:160-72.

Liu Ling-Zhen, Long Liu, Zhi-Hong Shi, Xian-Ling Bian, Zi-Ru Si, Qi-Quan Wang, Yang Xiang*, Yun Zhang*. Amphibian pore-forming protein βγ-CAT drives extracellular nutrient scavenging under cell nutrient deficiency.  Iscience . 2023;26(5). (WOS:001001126800001)

2022

Liu Ling-Zhen, Long Liu, Zhi-Hong Shi, Xian-Ling Bian, Qi-Quan Wang, Yang Xiang*, Yun Zhang*. Pore-forming protein βγ-CAT drives extracellular nutrient scavenging under cell starvation.  bioRxiv . 2022:2022.07.20.500773.

Zhang H, Qin J, Lan X, Zeng W, Zhou J, Huang TE, Xiao WL, Wang QQ, Sun S, Su W, Nie W, Yang S, Yang J, Gao Q, Xiang Y*. Handelin extends lifespan and healthspan of Caenorhabditis elegans by reducing ROS generation and improving motor function.  Biogerontology , 2022, 23 (1), 115-128. doi: 10.1007/s10522-022-09950-5. Epub ahead of print. PMID: 35038074. (WOS:000743394500001)

2020

Yang Ji, Huijie Zhang, Sujiao Sun, Xue Wang, Ying Guan, Qili Mi, Wanli Zeng, Haiying Xiang, Huadong Zhu, Xin Zou, Yang Xiang*, Qian Gao*. Autophagy and Hsp70 activation alleviate oral epithelial cell death induced by food-derived hypertonicity.  Cell Stress and Chaperones . 2020;25(2):253-64. (WOS:000518492000007)

2019

Xiang, Y., M. Zhao and Y. Zhang (2019). "Pore-forming toxin-like proteins link lysosomal function and cellular metabolism." Toxicon 158: S13. (WOS:000565208400045)

Zhao, M.-M., Z.-H. Shi, W.-H. Lee, Y. Xiang and Y. Zhang (2019). "The physiological function of aerolysin-like protein Lin-24 in Caenorhabditis elegans." Toxicon 158: S87-S88. (WOS:000565208400292)

2018

Xiang Yang*, Ming-ming Zhao, Sujiao Sun, Xiao-Long Guo, Qiquan Wang, Sheng-An Li, Wen-Hui Lee, Yun Zhang*. A high concentration of DMSO activates caspase-1 by increasing the cell membrane permeability of potassium.  Cytotechnology . 2018;70(1):313-20. (WOS:000424885900028)

2016

Sun Sujiao, Ping Jiang, Weiting Su, Yang Xiang*, Jian Li, Lin Zeng, Shuangjuan Yang. Wild chrysanthemum extract prevents UVB radiation-induced acute cell death and photoaging.  Cytotechnology . 2016;68(2):229-40.

2015

Yu Guoyu, Ping Jiang, Yang Xiang*, Yong Zhang, Zhu Zhu, Chuanrao Zhang, Siman Lee, Wenhui Lee, Yun Zhang*. Increased expression of protease-activated receptor 4 and trefoil factor 2 in human colorectal cancer.  PLoS One . 2015;10(4):e0122678.

Lei You-Ming, Yun-Fen Zu, Jiying Wang, Song Bai, Yun-Fei Shi, Rou Shi, Jin Duan, Daolei Cui, Jianlan Chen, Yang Xiang*, Jian Dong*. Interleukin-1β-mediated suppression of microRNA-101 and upregulation of enhancer of zeste homolog 2 is involved in particle-induced lung cancer. Medical Oncology. 2015;32(1):387.

2014

Xiang Yang, Chao Yan, Xiaolong Guo, Kaifeng Zhou, Qian Gao, Xuan Wang, Feng Zhao, Jie Liu, Wen-Hui Lee, Yun Zhang. Host-derived, pore-forming toxin–like protein and trefoil factor complex protects the host against microbial infection.  Proceedings of the National Academy of Sciences . 2014;111(18):6702-7. (当期封面文章，被Nature Reviews Microbiology和Aging Research Reviews正面引用)

Wang Yan-Jie, Xiao-Long Guo, Sheng-An Li, Yu-Qi Zhao, Zi-Chao Liu, Wen-Hui Lee, Yang Xiang*, Yun Zhang*. Prohibitin is involved in the activated internalization and degradation of protease-activated receptor 1.  Biochimica et Biophysica Acta (BBA)-Molecular Cell Research . 2014;1843(7):1393-401.

Zhao Feng, Chao Yan, Xuan Wang, Yang Yang, Guangyin Wang, Wenhui Lee, Yang Xiang*, Yun Zhang*. Comprehensive transcriptome profiling and functional analysis of the frog (Bombina maxima) immune system.  DNA research . 2014;21(1):1-13.

2013

Xiang Yang, Xuan Wang, Chao Yan, Qian Gao, Sheng-An Li, Jie Liu, Kaifeng Zhou, Xiaolong Guo, Wenhui Lee, Yun Zhang. Adenosine-5'-triphosphate (ATP) protects mice against bacterial infection by activation of the NLRP3 inflammasome.  PLoS One . 2013;8(5):e63759.

2012

Xiang Yang, Qian Gao, Weiting Su, Lin Zeng, Jinhuan Wang, Yi Hu, Wenhui Nie, Xutong Ma, Yong Zhang, Wenhui Lee, Yun Zhang. Establishment, characterization and immortalization of a fibroblast cell line from the Chinese red belly toad Bombina maxima skin.  Cytotechnology . 2012;64(1):95-105.

参与发表文章

2023

Ye Qingqing, Qiquan Wang, Wenhui Lee, Yang Xiang, Jixue Yuan, Yun Zhang, Xiaolong Guo. A Pore Forming Toxin-like Protein Derived from Chinese Red Belly Toad Bombina maxima Triggers the Pyroptosis of Hippomal Neural Cells and Impairs the Cognitive Ability of Mice.  Toxins . 2023;15(3):191.

Duan Yi, Yang Xiang, Jimin Chu, Xianghong Lin, Miao He, Chenggui Zhang, Sujiao Sun, Ling Huang. Handelin Reduces Ultraviolet A-Induced Photoaging by Inhibiting Reactive Oxygen Species Generation and Enhancing Autophagy.  The Tohoku Journal of Experimental Medicine . 2023;259(3):189-98. (WOS:000993309000003)

Chu Jimin, Yang Xiang, Xianghong Lin, Miao He, Yan Wang, Qiong Ma, Jingxian Duan, Sunjiao Sun. Handelin protects human skin keratinocytes against ultraviolet B-induced photodamage via autophagy activation by regulating the AMPK-mTOR signaling pathway.  Archives of Biochemistry and Biophysics . 2023:109646. (WOS:001013011200001)

2022

Long Changkun, Hongfei Liu, Wenxing Zhan, Liping Chen, Zhenping Yu, Shane Tian, Yang Xiang, Shenghan Chen, Xiao‐Li Tian. Chronological attenuation of NPRA/PKG/AMPK signaling promotes vascular aging and elevates blood pressure.  Aging cell . 2022:e13699. (WOS:000844435100001)

2020

Wang Qiquan, Xianling Bian, Lin Zeng, Fei Pan, Lingzhen Liu, Jinyang Liang, Lingyan Wang, Kaifeng Zhou, Wenhui Lee, Yang Xiang, Yun Zhang. A cellular endolysosome-modulating pore-forming protein from a toad is negatively regulated by its paralog under oxidizing conditions.  Journal of Biological Chemistry . 2020;295(30):10293-306. (WOS:000556713400016)

2019

Khan Abdul Haseeb, Zhiwen Zou, Yang Xiang, Shenghan Chen, Xiao-Li Tian. Conserved signaling pathways genetically associated with longevity across the species.  Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease . 2019;1865(7):1745-55. (WOS:000470042800003)

Guo Xiao-Long, Ling-Zhen Liu, Qi-Quan Wang, Jin-Yang Liang, Wen-Hui Lee, Yang Xiang, Sheng-An Li, Yun Zhang. Endogenous pore-forming protein complex targets acidic glycosphingolipids in lipid rafts to initiate endolysosome regulation.  Communications biology . 2019;2(1):1-13. (WOS:000461154000002)

2018

Zhao Feng, Xinqiang Lan, Tao Li, Yang Xiang, Fang Zhao, Yun Zhang, Wen-Hui Lee. Proteotranscriptomic analysis and discovery of the profile and diversity of toxin-like proteins in centipede.  Molecular & Cellular Proteomics . 2018;17(4):709-20.

2017

Li Sheng-An, Long Liu, Xiao-long Guo, Yu-Yan Zhang, Yang Xiang, Qi-Quan Wang, Wen-hui Lee, Yun Zhang. Host pore-forming protein complex neutralizes the acidification of endocytic organelles to counteract intracellular pathogens.  The Journal of infectious diseases . 2017;215(11):1753-63.

2014

Si-Man LI, Ping Jiang, Yang Xiang, WANG Wei-Wei, ZHU Yue-Chun, FENG Wei-Yang, LI Shu-De, YU Guo-Yu. Protease-activated receptor (PAR) 1, PAR2 and PAR4 expressions in esophageal squamous cell carcinoma.  Zoological Research . 2014;35(5):420.

Li Sheng-An, Jie Liu, Yang Xiang, Yan-Jie Wang, Wen-Hui Lee, Yun Zhang. Therapeutic potential of the antimicrobial peptide OH-CATH30 for antibiotic-resistant Pseudomonas aeruginosa keratitis.  Antimicrobial agents and chemotherapy. 2014;58(6):3144-50.

Li Sheng-An, Jie Liu, Yang Xiang, Yan-Jie Wang, Wen-Hui Lee, Yun Zhang. The antimicrobial peptide OH-CATH30 as a potential therapy for antibiotic-resistant Pseudomonas aeruginosa keratitis.  Antimicrobial Agents and Chemotherapy . 2014.

2013

Li Sheng-An, Yang Xiang, Yan-Jie Wang, Jie Liu, Wen-Hui Lee, Yun Zhang. Naturally occurring antimicrobial peptide OH-CATH30 selectively regulates the innate immune response to protect against sepsis.  Journal of medicinal chemistry . 2013;56(22):9136-45.

Jiang Ping, Guo-Yu Yu, Yong Zhang, Yang Xiang, Hai-Rong Hua, Li Bian, Chun-Yan Wang, Wen-Hui Lee, Yun Zhang. Down-regulation of protease-activated receptor 4 in lung adenocarcinoma is associated with a more aggressive phenotype.  Asian Pacific Journal of Cancer Prevention . 2013;14(6):3793-8.

Jiang Ping, Yang Xiang, Yan-Jie Wang, Si-Man Li, Yan Wang, Hai-Rong Hua, Guo-Yu Yu, Yong Zhang, Wen-Hui Lee, Yun Zhang. Differential expression and subcellular localization of Prohibitin 1 are related to tumorigenesis and progression of non-small cell lung cancer.  International journal of clinical and experimental pathology . 2013;6(10):2092.

Du Ting-yi, Hui-ming Luo, Hai-chun Qin, Fang Wang, Qing Wang, Yang Xiang, Yun Zhang. Circulating serum trefoil factor 3 (TFF3) is dramatically increased in chronic kidney disease.  PloS one . 2013;8(11):e80271.

2012

Zhang Yong, Yanjie Wang, Yang Xiang, W Lee, Y Zhang. Prohibitins are involved in protease‐activated receptor 1‐mediated platelet aggregation.  Journal of Thrombosis and Haemostasis . 2012;10(3):411-8.

2011

Zhang Yong, Guoyu Yu, Yanjie Wang, Yang Xiang, Qian Gao, Ping Jiang, Jie Zhang, Wenhui Lee, Yun Zhang. Activation of protease-activated receptor (PAR) 1 by frog trefoil factor (TFF) 2 and PAR4 by human TFF2.  Cellular and molecular life sciences . 2011;68(22):3771-80.

Zhang Yong, Guoyu Yu, Ping Jiang, Yang Xiang, Wenliang Li, Wenhui Lee, Yun Zhang. Decreased expression of protease-activated receptor 4 in human gastric cancer.  The international journal of biochemistry & cell biology . 2011;43(9):1277-83.

Gao Qian, Yang Xiang, Lin Zeng, Xu Tong Ma, Wen Hui Lee, Yun Zhang. Characterization of the βγ-crystallin domains of βγ-CAT, a non-lens βγ-crystallin and trefoil factor complex, from the skin of the toad Bombina maxima.  Biochimie . 2011;93(10):1865-72.

Gao Qian, Yang Xiang, Zhongming Chen, Lin Zeng, Xutong Ma, Yun Zhang. βγ-CAT, a non-lens betagamma-crystallin and trefoil factor complex, induces calcium-dependent platelet apoptosis.  Thrombosis and haemostasis . 2011;105(05):846-54.

2010

Zhang Yong, Guoyu Yu, Yang Xiang, Jianbo Wu, Ping Jiang, Wenhui Lee, Yun Zhang. Bm-TFF2, a toad trefoil factor, promotes cell migration, survival and wound healing.  Biochemical and biophysical research communications . 2010;398(3):559-64.

Yu Guoyu, Yong Zhang, Yang Xiang, Ping Jiang, Zhongming Chen, Wenhui Lee, Yun Zhang*. Cell migration-promoting and apoptosis-inhibiting activities of Bm-TFF2 require distinct structure basis.  Biochemical and biophysical research communications . 2010;400(4):724-8.

参编书籍

老年医学，2020，人民卫生出版社（卫健委十三五规划教材），北京.

中国动物多肽毒素，2016，科学出版社，北京.

教学工作

本科生课程《生物化学》

博士生双语课程《Molecular and Cellular Biology》

联系方式

地址：江西省南昌市红谷滩新区学府大道999号南昌大学理科生命大楼人类 衰老研究所一楼。

联系方式：xiangyang@ncu.edu.cn; xiangyanghari@163.com

团队成员

兰新强 助理研究员 lanxinqiang@ncu.edu.cn

王齐权 博士后   wangqiquan@ncu.edu.cn

在读博士生：肖婉莉、黄天鹅、王翔

在读硕士生：黄春萍、王佳丽、徐思曼、杨梦琪、姚梦、刘彦、赵笑霜

毕业博士生：张慧杰

毕业硕士生：尤云菲、朱华栋、秦嘉蔚、邹鑫、周晶、曾维荣、汪本辉